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Immunohistochemical staining for CD34 and α-SMA in the EL of the proximal ACL in the human knee. ( A , B ): Immunohistochemical staining for CD34. ( A ) Scale bar, 100 μm; ( B ) Scale bar, 50 μm; ( C , D ). Immunohistochemical staining for α-SMA. ( C ) Scale bar, 100 μm; ( D ) Scale bar, 50 μm.

Journal: Biomedicines

Article Title: Do the Differences in the Epiligament of the Proximal and Distal Parts of the Anterior Cruciate Ligament Explain Their Different Healing Capacities? Quantitative and Immunohistochemical Analysis of CD34 and α-SMA Expression in Relation to the Epiligament Theory

doi: 10.3390/biomedicines12010156

Figure Lengend Snippet: Immunohistochemical staining for CD34 and α-SMA in the EL of the proximal ACL in the human knee. ( A , B ): Immunohistochemical staining for CD34. ( A ) Scale bar, 100 μm; ( B ) Scale bar, 50 μm; ( C , D ). Immunohistochemical staining for α-SMA. ( C ) Scale bar, 100 μm; ( D ) Scale bar, 50 μm.

Article Snippet: The following antibodies were used for immunohistochemistry: monoclonal mouse anti-human α-SMA (M0851, DAKO, Agilent Technologies, Inc., Santa Clara, CA, USA) and monoclonal mouse anti-human CD34 (M7165, DAKO, Agilent), both diluted 1:100.

Techniques: Immunohistochemistry, Staining

Immunohistochemical staining for CD34 and α-SMA in the EL of the distal ACL in the human knee. ( A , B ) Immunohistochemical staining for CD34. ( A ) Scale bar, 100 μm; ( B ) Scale bar, 50 μm; ( C , D ). Immunohistochemical staining for α-SMA. ( C ) Scale bar, 100 μm; ( D ) Scale bar, 50 μm.

Journal: Biomedicines

Article Title: Do the Differences in the Epiligament of the Proximal and Distal Parts of the Anterior Cruciate Ligament Explain Their Different Healing Capacities? Quantitative and Immunohistochemical Analysis of CD34 and α-SMA Expression in Relation to the Epiligament Theory

doi: 10.3390/biomedicines12010156

Figure Lengend Snippet: Immunohistochemical staining for CD34 and α-SMA in the EL of the distal ACL in the human knee. ( A , B ) Immunohistochemical staining for CD34. ( A ) Scale bar, 100 μm; ( B ) Scale bar, 50 μm; ( C , D ). Immunohistochemical staining for α-SMA. ( C ) Scale bar, 100 μm; ( D ) Scale bar, 50 μm.

Article Snippet: The following antibodies were used for immunohistochemistry: monoclonal mouse anti-human α-SMA (M0851, DAKO, Agilent Technologies, Inc., Santa Clara, CA, USA) and monoclonal mouse anti-human CD34 (M7165, DAKO, Agilent), both diluted 1:100.

Techniques: Immunohistochemistry, Staining

Percentages of overall image grades, according to ICH Profiler-generated scores for CD34 and α-SMA in the EL of the ACL in the human knee. Bars are color-coded according to the immunohistochemical staining: CD34—orange, α-SMA—green. Top row—distal EL images; bottom row—proximal EL images.

Journal: Biomedicines

Article Title: Do the Differences in the Epiligament of the Proximal and Distal Parts of the Anterior Cruciate Ligament Explain Their Different Healing Capacities? Quantitative and Immunohistochemical Analysis of CD34 and α-SMA Expression in Relation to the Epiligament Theory

doi: 10.3390/biomedicines12010156

Figure Lengend Snippet: Percentages of overall image grades, according to ICH Profiler-generated scores for CD34 and α-SMA in the EL of the ACL in the human knee. Bars are color-coded according to the immunohistochemical staining: CD34—orange, α-SMA—green. Top row—distal EL images; bottom row—proximal EL images.

Article Snippet: The following antibodies were used for immunohistochemistry: monoclonal mouse anti-human α-SMA (M0851, DAKO, Agilent Technologies, Inc., Santa Clara, CA, USA) and monoclonal mouse anti-human CD34 (M7165, DAKO, Agilent), both diluted 1:100.

Techniques: Generated, Immunohistochemistry, Staining